大青叶中靛玉红的超临界萃取工艺及含量测定

SFE technology of indirubin from Daqingye and content determination of indirubin

目的建立用HPLC测定大青叶的超临界萃取物中靛玉红含量的方法,对萃取工艺进行优选.方法采用超临界萃取法进行L9(34)正交实验.以靛玉红含量考察萃取温度、萃取压力、分离温度及分离压力四个因素的影响.采用HPLC法测定萃取物中靛玉红含量.结果最佳萃取工艺为萃取温度50℃,萃取压力40MPa,分离温度40℃及分离压力8MPa.靛玉红平均回收率为102.8%,RSD为1.48%.结论萃取温度、萃取压力及分离压力对大青叶中靛玉红的萃取都有显著影响.HPLC法操作简便,结果准确,可作为超临界萃取大青叶中靛玉红的质量控制方法.

AIM： To use HPLC for the content determination of indirubin in supercritical fluid extraction （SFE） material from Daqingye and to optimize the process of SFE. METHODS： Content of indirubin was used as index to evaluate the technologies based on orthogonal design, in which 4 factors considered were extraction temperature and pressure, separation temperature and pressure. A HPLC system was used to determine the content of indirubin. RESULTS： Optimal program was as follows： extraction temperature 50℃ and pressure 40 MPa, separation temperatore 40℃ and pressure 8 MPa. The average recovery of indirubin was 102. 8% and RSD was 1. 48%. CONCLUSION： The extraction temperature, extraction pressure and separation pressure all have significant effects on the extraction rate of indirubin. The HPLC method is simple, precise and suitable for the content determination of indirubin in SFE material of Daqingye .