癌间质成纤维细胞对口腔鳞癌细胞HLA-A和CD86表达的影响

Stromal fibroblasts down-regulates the transcription and expression of HLA-A and CD86 in oral squamous cell carcinoma

目的:研究肿瘤间质成纤维细胞(TSF)对肿瘤细胞人白细胞抗原A(HLA-A)和CD86转录和表达的影响。方法:将口腔鳞癌细胞系Tca-8113细胞与口腔TSF共培养,采用RT-PCR、流式细胞术和Western blotting等方法检测HLA-A,HLA-B,HLA-G和CD86的转录及表达。结果:Tca-8113细胞HLA-A和CD86的转录与TSF共培养后下调,分别只有对照组Tca-8113的66.49%和52.31%,差异均有统计学意义(P<0.05),其表达也有相同的结果;但HLA-B和HLA-G未受影响。结论:TSF下调口腔鳞癌细胞HLA-A和CD86的转录和表达,造成肿瘤的免疫逃逸,有助于肿瘤的转移和演进。

Objective：To study the role of tumor-stromal fibroblasts（TSF） on the transcription and expression of human leukocyte antigen A （HLA-A） and CD86. Methods： Tca-8113 cells （oral tongue squamous carcinoma cell line） were co-cultivated with TSF in a noncontact manner by Millicell chamber, and then the transcription and the expression of HLA-A, HLA-B, HLA-G and CD86 of Tca-8113 cells were detected by semi-quantitive RTPCR, Western blotting and flow cytometry analysis. Results： The transcripts of HLA-A and CD86 of Tca-8113 were down-regulated by TSF to 66.49% and 52.31%, respectively compared with the control （P 〈 0.05）; But HLA-B and HLA-G were not influenced almost. Conclusion： TSF down-regulates the expression of HLA-A and CD86 in oral squamous cells, and therefore the escape from immune may result in tumor progression and metastasis in vivo.