己酮可可碱对大鼠胰腺纤维化的影响

Effect of pentoxifylline on pancreas fibrosis in rat

目的:观察己酮可可碱(PTX)对大鼠胰腺纤维化的影响。方法:大鼠胰管内注射2%三硝基苯磺酸(TNBS),制备大鼠胰腺纤维化模型。将大鼠12只随机分为PTX干预组、对照组。PTX干预组于术后第2天给予PTX 6 mg/(kg.d)腹腔注射,4周后收集两组胰腺标本。采用免疫组化技术检测治疗前后胰腺组织中α-平滑肌肌动蛋白(-αSMA)、血小板衍化生长因子(PDGF)、Ⅰ型胶原(COLⅠ)、磷酸化细胞外信号调节激酶1(P-ERK1)的含量同时做组织胶原纤维染色,部分胰腺组织液氮冻存,以Western blot方法检测-αSMA的表达。结果:对照组发生胰腺纤维化,PTX干预组有轻度纤维化。PTX干预组-αSMA、PDGF、COLⅠ的含量以及P-ERK1在基质细胞中的表达均低于对照组(P<0.01)。胶原纤维染色显示,PTX治疗组胶原面积百分比明显低于对照组(P<0.01)。结论:PTX有抗大鼠胰腺纤维化的作用。

Objective： To observe the effects of pentoxifylline on pancreas fibrosis in rat. Methods： TNBS in a concentration of 2% was injected into the rats＇pancreatic duct to set up the model of pancreatitis. Rats of interventional group were treated with FIX, and the expression of PDGF, α-SMA, COL Ⅰ and P-ERK1 were determined by using immunohistochemistry technique and HE and Masson staining in some samples 4 weeks later, meanwhile the expression of α-SMA was detected by using Western blotting. Results： Pancreas fibrosis was observed in control group, whereas it was not significant in FIX treatment group. Compared with the control, FIX significantly reduced both the content of α-SMA, PDGF, COL Ⅰ and P-ERK1 （ P 〈 0.01）and the pancreas fibrosis proportion（ P 〈 0.01）. Conclusion： Pancreas fibrosis can be inhibited by FIX in rats.