手术前应用七叶皂苷钠对大鼠脊髓牵拉损伤后的行为及诱发电位的影响

Influence of sodium aescinate on locomotion function and somatosensory evoked potentials in rats

目的:通过观察大鼠脊髓牵拉损伤后的行为、体感诱发电位变化的规律,评价手术前应用七叶皂苷钠对脊髓功能的影响。方法:取成年Wistar大鼠80只,随机分为实验组和对照组。实验组手术前30 min尾静脉注射七叶皂苷钠60 mg/kg,切除胸10全椎板,将脊髓水平方向牵拉1.7 mm,持续5 min,造成脊髓牵拉损伤模型。分别于手术后24 h3、d7、d、14 d处死,对前后肢进行BBB评分、通过斜板试验评价后肢运动功能;记录体感诱发电位评价感觉功能恢复状况。结果:①牵拉脊髓水平位移1.7 mm,持续5 min造成大鼠不完全性双后肢瘫痪;②损伤后3 d,BBB功能评分、斜板试验、体感诱发电位的潜伏期,实验组与对照组的差异有统计学意义(P<0.05);7 d1、4 d时运动功能评分、体感诱发电位两组的差异有统计学意义(P<0.01)。结论:体感诱发电位能较准确地反映脊髓损伤情况和功能恢复的情况。手术前应用七叶皂苷钠有利于术后动物脊髓功能的恢复。

Objective：To observe the changes of behavior functional tests and somatosensory evoked potentials after tractive spinal cord injury （SCI）in rats, and evaluate the influence of sodium aescinate （SA） on spinal cords function. Methods： Eighty Wistar rats were divided into experimental groups and control groups randomly. SA in a dose of 6 mg/kg was intravenously injected into the tail of the experimental rat, and 30 minutes later, whose spinal cord was exposed entirely via dual laminectomy at T10 level and pulled horizontally （displacement of 1.7mm, duration of 5minutes） by a special retractor imitating the neural ecarteur to make the SCI model. The behavior functional tests of hind limbs of rats were evaluated by BBB locomotion scores and inclined plane, and the sensory function ofrats was monitored by cortical somatosensory evoked potential （CSEP） test at different time-point after SCI. Results： （1） Incomplete paralysis of rats was established by pulling spinal cord for 1.7mm and 5min horizontally; （2） BBB scores, inclined plane test and latency of CSEP showed significantly different between the two groups in 3 days, and behavior functional tests and CSEP were significantly different between the two in 7 and 14 days. Conclusion： CSEP examination provides an objective and quantitative standard to judge the sensory functional status of spinal cords injury. Preoperative injection of high dose SA has salutary effect on the recovery of SCI.