钟花樱组织培养再生体系的建立

Regeneration System of Tissue Culture for Cerasus cerasoides var. campanulata

对钟花樱组织培养再生体系进行研究,结果表明,BA／NAA约为10时,即取BA 0．8-1．0mg／L,NAA0．08- 0．1mg/L(单位下同),对芽的诱导有较好的效果,培养基组合为MS+BA 1．0+NAA0．1。在芽的增殖与伸长方面,筛选出较优的培养基组合为1／2MS+6-BA 1．0+NAA0．1+GA30．5+肌醇25,芽的增殖系数为4．7,芽均高1．314 cm。在此基础上通过5次连续继代培养,芽的增殖系数达到了7．31,丛生芽诱导率81．0％。钟花樱根的诱导试验表明, 通过暗培养3 d,以1／2MS+NAA 0．2+IBA 0．8+6-BA 0．01的培养成份组合最适宜根的诱导,生根率达90％。试验中低浓度的细胞分裂素6-BA对提高生根率是必要的。

The regeneration system of Cerasus cerasoides var. campanulata was developed in this paper. The results indicated that the hormone combination in the culture medium had obvious effects on bud induction. Especially when the concentration ratio of BA and NAA is approximately 10, for example, BA 0.8 - 1.0mg/L, NAA 0.08 - 0. ling/L, bud induction could be better. The optimal medium for bud induction is MS ＋ BA1.0 mg/L ＋ NAA0.1 mg/L, while for bud proliferation and bud elongation, the appropriate medium is 1/2MS ＋ 6 - BA1.0 mg/L ＋ NAA0.1 mg/L ＋ GA30.5 mg/L ＋ Inositol 25 mg/L. Under this culture condition, the bud proliferation coefficient could reach 7.31, and cluster buds inducement rate 81.0%. The appropriate root induction medium is 1/2MS＋ NAA0.2 mg/L ＋ IBA0.8 mg/L ＋ 6- BA0.01 mg/L, the rooting rate could reach 90% after 3 days dark culture in this medium. It was also found that low concentration of 6 - BA is essential for root inducement.