摘要
以托柄菝葜幼嫩的茎段为外植体,MS、1／2MS、1／4MS、B5为基本培养基,研究了不同植物激素组合对托柄菝葜组织培养快速繁殖的影响。试验结果表明,不同培养阶段的适宜培养基为:(1)启动培养基为1／2MS+6-BA 2．0 mg／L+IBA 0．1 mg／L;(2)继代增殖培养基为1／2MS+6-BA 3．0 mg／L+IBA 0．2 mg／L,月平均芽增殖率可达 177．8％;(3)生根培养基为1／4MS+NAA 1．5 mg／L+活性炭1 000 mg／L,培养25 d后生根率可达93．3％。
Taking the young stem of Smilax discotis Warb, as the explant,using the MS, 1/2MS, 1/4MS,B5 as the basic culture medium,the effects of hormone combination on callus formation and plantlet regeneration of Smilax discotis Warb. were studied in this paper. The results indicated that the suitable culture medium at different culture stages was： （ 1 ） Initial medium： 1/2MS ＋ 6 - BA2.0mg/L ＋ IBA0. ling/L; （2） Proliferation medium. 1/2MS ＋ 6- BA3.0mg/L ＋ IBA0.2mg/L, （3）Rooting medium： 1/4MS ＋ NAA1.5mg/L＋ activated carbon 1 000mg/L. The monthly average bud proliferation rate could reach 177.8% in proliferation medium, and the rooting ratio could reach 93.3% when cultivated in rooting medium for 25d.
出处
《林业科技开发》
2006年第3期42-45,共4页
China Forestry Science and Technology
关键词
托柄菝葜
组织培养
茎段
外植体
褐化
Smilax discotis Warb.
Tissue culture
Stem segment
Explant
Browning
