欧洲黑杨基因资源稳定碳同位素组成特征

Study on Stable Carbon Isotopic Composition of Populus nigra L.Gene Resource

通过测定从欧洲不同地区引进的134个欧洲黑杨无性系叶片的稳定碳同位素比率(δ13C值),研究了欧洲黑杨基因资源1δ3C值的特点和水分利用状况。结果表明:(1)引进的欧洲黑杨基因资源叶片δ13C值为-30.40‰^-27.02‰,水分利用效率具有丰富的多样性,优于国内乡土树种,其中来自俄罗斯、土耳其和德国的基因资源1δ3C值明显高于其它国家的基因资源,可为抗旱杨树新品种的选育提供优良的亲本材料;(2)相同基因资源在不同地区栽植时,δ13C值受环境的影响较大。生长在内蒙古地区的欧洲黑杨无性系叶片平均δ13C值为-26.53‰,明显高于在陕西和北京地区测得的-28.39‰和-28.93‰,故在进行不同基因型的δ13C值评价和选择时要在相同条件下进行;(3)生长在不同地区的欧洲黑杨的δ13C值与生长量的相关程度不同,生长在北京地区的欧洲黑杨δ13C值与株高、地径呈极显著正相关(P=0.01),相关系数分别为0.912和0.829,而生长在陕西和内蒙古地区的欧洲黑杨的δ13C值与生长指标虽然也相关,但达不到显著水平。在研究植物生长与水分利用效率之间的关系时,必须结合植物的生长环境进行分析。

Foliar δ^13C values as the indicating value of water use efficiency （WUE） were measured in 134 clones of Populus nigra L. introduced from different areas of Europe. We investigated the characteristics of δ^13C value and water use efficiency of gene resources. The foliar δ^13C value of P. nigra L. was abundant in diversity, ranging from - 30.40%0 to - 27.02%0. The difference of δ^13C value in gene resources from different areas was significant, The clones introduced from Russia ,Turkey and Germany had higher δ^13C value and could be used as parents for breeding new variety . The δ^13C value of same clones planted in different areas of China was affected by environment greatly,δ^13C value of P. nigra L. clones planted in Inner Mongolia was -26.53%0, which was higher than those planted in Beijing and Shaanxi. The correlation between δ^13C value and growth was different when P. nigra L. were planted in different areas. The correlation coefficients between δ^13C and plant height, between δ^13C and ground diameter of clones in Beijing were 0. 912 and 0. 829 respectively, whereas between δ^13C value and growth of clones in Inner Mongolia and Shaanxi showed no significant correlation. Therefore, it is necessary to compare the δ^13C value of different genotype in the same growth condition and analyze the correlation between δ^13C value and growth status for future scanning and breeding the new variety with high WUE and high productivity.