胃移停颗粒对S_(180)小鼠抑瘤作用及其对P^(53)和PCNA表达的影响

Antitumor Activity of Wei Yi Ting Granules on S_(180) Sarcoma Mice and Its Influence on the Expression of P^(53) and PCNA

目的探讨胃移停颗粒的抑瘤作用和可能机制。方法观察胃移停颗粒及其拆方对S180肉瘤小鼠的抑制作用,并用免疫组化的方法研究胃移停颗粒及其拆方在S180肉瘤生长过程中对P53、PCAN表达的影响。结果胃移停颗粒抑瘤作用明确,且能显著降低P53、PCAN阳性细胞数。胃移停颗粒与其拆方各组比较,除益气活血解毒组无显著性差异(P>0.05),其余两组差异有显著性意义(P<0.05～0.01)。结论以益气活血解毒立法处方抑瘤作用确切,较益气组和益气解毒组作用明显;胃移停颗粒能抑制P53和PCNA的表达,对肿瘤生长过程中肿瘤细胞的基因水平有影响作用。

Objective To observe the antitumor action of Wei Yi Ting granules （WYTG） and to explore its possible mechanism. Methods The inhibitory effect of WYTG and its different WYTG ingredient combinations on the growth of S180 tumor in mice was observed. Meanwhile, the effect of WYTG and its different ingredient combinations on the expression of P^53 and PCAN during tumor development was studied by immunohistochemical method. Results WYTG had an obvious inhibitory effect on tumor growth and could reduce the positive cell number of P^53 and PCAN, the difference being significant as compared with the blank control group （ P 〈 0.05）. Compared with its different ingredient combinations, the difference was significant in WYTG group （ P 〈 0.05 - 0.01 ） except that in the ingredient combination of strengthening Qi, activating blood and removing toxicity （ P 〉 0.05） . Conclusion WYTG, which has the actions of strengthening Qi, activating blood and removing toxicity, exerts an obvious inhibitory effect on tumor as compared with its ingredient combination of strengthening Qi and with that of strengthening Qi and removing toxicity. Its therapeutic mechanism may be related to the inhibition of the expression of P^53 and PCAN and the influence on tumor - associated gene during the growth of tumor.