SD大鼠及beagle犬肝组织形态计量研究

Morphometric Analysis of Liver Tissue in SD Rats and Beagle Dogs

目的了解SD大鼠及beagle犬肝组织学参数,给毒理病理诊断提供组织学判断依据。方法以40只成年SD大鼠及20只成年beagle犬为研究对象,雌雄各半,大鼠在12周龄处死,beagle犬在12月龄处死。以10%中性甲醛固定肝脏,石蜡包埋,5μm切片,HE染色。以SpotAdvanced图像分析软件测量犬及大鼠中央静脉-汇管区以及相邻肝小叶中央静脉间距离,同时比较种属间及雌雄性动物之间的差别。结果beagle犬相邻肝小叶中央静脉间距大约为883μm,中央静脉-汇管区间距约为511μm,雌雄犬无差异。SD大鼠相邻肝小叶中央静脉间距约为496μm,而相邻肝小叶中央静脉-汇管区间距约为410μm,雌雄大鼠间无显著性差异。结论beagle犬肝小叶中央静脉间距大于SD大鼠肝小叶中央静脉间距;beagel犬中央静脉和汇管区间距同样大于SD大鼠的中央静脉-汇管区间距。犬及SD大鼠的性别不影响肝小叶大小。

Objective To investigate the parameters of liver tissue in SD rats and beagle dogs and to supply evidence for toxicologic pathological evaluation. Methods Forty SD rats and twenty beagle dogs, male in half, were used for the observation. SD rats were sacrificed at the age of 12 weeks and the beagles at the age of 12 months. After complete necropsy examination, all livers were fixed in 10 % neutral buffered formalin solution, embedded in paraffin, sliced at a thickness of 5 μm and stained with hematoxylin and eosin （HE）. The distance of central vein and portal area, and that of central veins in the neighbored lobules were measured by means of an image analyzer Spot Advanced. The difference of the above distance between beagles and rats, and that between male and female were compared. Results The distance of central veins in the neighbored lobules was about 883 μm in the beagles and about 496 μm in SD rats, and the distance between lobule center and portal area was about 511μm in beagle dogs and about 410 μm in SD rats. There was no difference between the male and the female. Conclusion The distance of central veins in the neighbored lobules in beagle dogs is longer than that in SD rats, so does the distance between lobule center and portal area. The sex of the animal has no influnce on the size of the lobules.