不同采收期陆英中绿原酸含量的动态研究

Determination of Chlorogenic Acid from Sambucus chinese in Various Collecting Periods

目的研究不同采收期陆英中绿原酸的含量变化,为确定陆英药材最佳采收期提供依据。方法采用RP-HPLC法测定不同采收期陆英药材中绿原酸的含量。色谱条件:Hypersil-ODS2色谱柱(250mm×4.6mm,5μm);流动相:乙腈-1%甲酸水溶液(9∶91);流速1.0mL.min-1;柱温35℃;检测波长326nm。结果不同采收期陆英药材中绿原酸含量以5月份为高,即开花前期;对于同一批药材的不同部位进行比较,叶中绿原酸含量明显高于茎、根。结论建议陆英药材在开花前枝叶繁盛时采收。

Objective To supply evidence for determining the optimal collecting period for Sambucus chinese. Methods A RP - HPLC method was established to determine chlorogenic acid contents from Sambucus chinese in various collecting periods. The chromatographic conditions were as follows： Hypersil - ODS2 （250 mm × 4. 6 mm, 5 μm）column, acetonitrile - 1% formic acid aqueous solution （9 ： 91 ） as mobile phase with a flow rate of 1.0 mL · min^-1, column temperature at 35 ℃and the detection wavelength at 326 nm. Results Content of chlorogenic acid in the samples collected in May （pre -blossom period）was the highest and the content in leaf was higher than in the stem and root. Conclusion It is suggested that Sambucus chinese should be gathered before blooming when the leaves are in luxuriance.