HPLC法测定鲜益母草中盐酸水苏碱含量

Determinatin of Stachydrine Hydrochloride in Fresh Medicinal Material of Herba Leonuri by HPLC

目的建立测定鲜益母草药材中盐酸水苏碱含量的方法。方法采用HPLC法,色谱柱为氨基色谱柱(250mm×4.6mm,5μm);流动相为乙腈-水(80∶20);流速为1.0mLm/in;紫外检测波长192nm;柱温22℃。供试品溶液以乙醇为洗脱液,经活性炭-中性氧化铝层析柱过滤净化。结果选用氨基柱,盐酸水苏碱成分可得到较好的分离,盐酸水苏碱在2.29～11.46μg范围内,进样量与峰面积呈良好的线性关系,平均回收率为98.26%,RSD为1.52%(n=5)。结论该方法稳定、重现性好,可作为鲜益母草药材中盐酸水苏碱的定量测定方法。

Objective To establish a HPLC method for the separation and determination of stachydrine hydrochloride in fresh medicinal material of Herba Leonuri. Methods The NH2 column（250 mm × 4.6 mm, 5 μm） was adopted with acetonitrile - water（80 ： 20） as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was at 192 nm. The column temperature was at 22 ℃. The solution for content determination was obtained after extracted with ethanol and then filtered and purified through an activated charcoal-aluminium oxide column with ethanol as eluant. Results Stachydrine hydrochloride in fresh Herba Leonuri can be separated and determined on NH2 column. A good linear range was within 2.29 - 11.46μg. The average recovery for stachydrine hydrochloride was 98.26 % and RSD was 1.52 % （ n = 5） . Conclusion The method is steady and with good repeatability, and can be used to determine the content of stachydrine hydrochloride in fresh Herba Leonuri.