HPLC法测定芩黄喉症胶囊中大黄素的含量

Determination of the Content of Emodin in Qinhuang Houzhen Capsule by HPLC

目的测定芩黄喉症胶囊中大黄素的含量,为该制剂制定质量标准提供依据。方法采用HPLC法测定,色谱条件:DionexAcclaimTM120C18柱(4.6mm×150mm,5μm);流动相:甲醇-0.4%磷酸溶液(85∶15);流速:1.0mLm/in;检测波长:254nm。结果大黄素在0.12～1.92μg之间呈线性关系,r=0.9999;平均回收率为97.5%,RSD=2.29%(n=5);该制剂中大黄素平均含量为0.2057mgg/,RSD=4.11%。结论该方法简便准确,重现性好,可用于芩黄喉症胶囊的质量控制。

Objective To determine the content of emodin in Qinhuang Houzhen Capsule and to provide evidence for its quality criteria. Method A HPLC method was established as follows： Dionex AcclaimTM120 C18 cloumn（4.6 × 150 mm, 5 μm）, methnol- 0. 4 % phosphoric acid aqueous solution （85： 15） as mobile phase, flow rate at 1.0 mL/min and detection wavelength at 254 nm. Results The average content of emodin in Qinhuang Houzhen Capsule was 0. 2057 mg/g, and RSD = 4. 11 %. Conclusion The content of rhubarb calculated by emodin in 1 g Qinhuang Houzhen Capsule could be affirmed at 0. 150 mg/g tentatively. The method is simple, acurate, repeatible, and can be used for the quality control of Qinhuang Houzhen Capsule.