摘要
目的研究抑癌基因PTEN对乳腺癌细胞转移的影响。方法脂质体介导法将外源野生型抑癌基因PTEN转染入该基因缺陷的ZR-75-1乳腺癌细胞中,用嘌呤霉素筛选阳性克隆,Western-blot法检测PTEN蛋白表达;重组人工基底膜上检测黏附与侵袭能力。结果转染后ZR-75-1乳腺癌细胞PTEN蛋白有明显表达;转染后ZR-75-1乳腺癌细胞侵袭抑制率与黏附抑制率分别达70.4%和60.0%。结论PTEN基因对乳腺癌细胞转移具有一定的抑制作用;PTEN基因的缺失与否在一定程度上可以评价乳腺癌患者发生转移的危险程度。
Objective To disclose the influence of PTEN on the migration of breast cancer cells ZR-75-1. Methods Wild-type PTEN gene was transtected into ZR-75-1 breast cancer cells which lack PTEN gene, tranfected cells were selected by puromycin and the protein encoded by PTEN gene was tested by Western-blot. The inhibition rate of invasion and adhesion of ZR-75-1 was tested on reconstitituted basement membrane. Results Wild-type PTEN gene was successfully transfected into ZR-75-1 and expressed efficiently. The inhibition rate of invasion and adhesion is 70.4 % and 60.0 % respectively. Conclusion PTEN gene can restrict the migration of breast cancer cells in some degree, so whether PTEN gene is deleted or not can partly estimates the risk of migration of breast cancer cells.
出处
《肿瘤研究与临床》
CAS
2006年第5期296-297,300,共3页
Cancer Research and Clinic
基金
湛江市科技招标资助项目(GK0302)
