L-苯丙氨酸衍生物的制备及其固定化

Preparation of L-Phenylalanine Derivative and Its Fixiation on Silica Gel Surface

将L-苯丙氨酸分别与对甲苯磺酰氯、苯甲酰氯反应得到两种不同的衍生物,再分别转化为酰氯,并与氨丙基硅胶反应制备成新型手性固定相,用元素分析和红外光谱对衍生物和手性固定相进行了表征,用L-苯丙氨酸的对甲苯磺酰氯衍生物制成的手性固定相对氯霉素进行了手性拆分。

Two L-phenylalanine derivatives were prepared by the reaction between L-phenylalanine and ptoluenesulfonyl chloride or benzoyl chloride. After converting into their acyl chloride,these two derivatives reacted with 3-aminopropyl silica gel to give CSP1 and CSP2. The derivatives and CSPs had been characterized by elemental analysis and FT-IR. CSP1 was packed into stainless steel column,and this chiral column was used to separate chloramphenicol by high performance liquid chromatography. The corresponding chromatogram demonstrated a satisfactory enantioseparation was achieved.