组织工程技术尿道重建的实验研究

Experimental research on urethral reconstruction with tissue engineering

目的利用组织工程技术进行人工管状皮肤的培养,探讨其用于尿道重建的可行性。方法用Wistar大鼠乳鼠皮肤为细胞来源,结合管状壳多糖基质网架,在体外共同培养成复层皮肤结构,移植到裸鼠皮下。分别于移植的5、7、14、28、35d收取标本,光镜下观察移植物生长情况。以没有种植细胞的壳多糖基质网架作为对照组,进行对比分析。结果移植物在裸鼠皮下生长良好,未发生收缩。移植初期细胞数量少,排列不规律;随着时间的延长,细胞数量逐渐增多,排列趋于规律。所有移植物均见成纤维细胞生长,约5-7层,细胞呈长梭形,成纤维细胞向表皮细胞层游走,并规律的生长在表皮细胞层下,细胞大致按层生长,只有少量细胞相互长入对方。在移植后的5d,可见有毛细血管长入。之后,毛细血管的数目日渐增多。移植物的表皮层生长良好,呈4-6层,但分化较差。对照组可见有大量炎性细胞。结论移植物的成纤维细胞生长、分化较好,表皮细胞增殖较好,但分化差。以壳多糖为支架的人工皮肤进行尿道修复具有可行性。

Objective To evaluate the feasibility of urethral reconstruction with the tissue engineered artificial epithelium. Methods The epithelial cells, which were taken from neonate Wistar rat, were cultured into tubiform epithelium together with the frame of shell polysaccharide matrix in vitro. The tubiform epithelium was transferred into subcutis of nude mice, and harvested for histologic study on day 5,7,14,28 and 35. As contrast, the shell polysaccharide matrix frame without cells was transplanted in the control group. Results Transplanted tubiform epithelium grew very well and no shrink was found. There were capillaries in the epithelium 5 days after transplantation. There were a fair amount of phlegmonosis cells in the area of shell polysaccharide matrix in the control group. Conclusion With the help of shell polysaccharide matrix frame, tissue engineered tubiform epithelium was cultured successfully. There is possibility to reconstruct urethra with tissue engineeing.