转化生长因子β_1对巨噬细胞纤溶酶原激活物抑制因子-1表达的影响

Influence of transforming growth factor-β_1 on plasminogen activator inhibitor-1 expression in RAW 264.7 cells

①目的研究转化生长因子β1(TGF-β1)对RAW 264.7细胞纤溶酶原激活物抑制因子-1(PAI-1)表达的影响。②方法以TGF-β1(5ng/m l)刺激RAW 264.7细胞,分别在作用0、12、24、48小时后,采用免疫细胞化学SP法、W estern印迹以及酶联免疫吸附实验(ELISA)观察细胞PAI-1表达的变化。③结果TGF-β1刺激RAW 264.7细胞,0小时组、12小时组、24小时组、48小时组的PAI-1免疫染色强度积分分别为36.23±7.35、62.23±11.18、98.58±4.98、130.42±8.56,PAI-1蛋白相对灰度值分别为0.85±0.05、1.21±0.06、1.56±0.08、2.43±0.02,细胞上清液中PAI-1含量(ng/mL)分别为15.23±1.98、26.35±2.93、40.21±1.54、69.32±3.21。④结论TGF-β1能够诱导RAW 264.7细胞PAI-1的表达,并存在明显的时效关系。

Objective To study the influence of transforming growth factor - β1 （ TGF - β1 ） on plasminogen activator inhibitor - 1 （PAI - 1 ） expression in RAW264.7 cells. Methods RAW264.7 cells were incubated with 5ng/ml TGF - β1 for Oh, 12h, 24h, 48h. The PAI- 1 expression was investigated by SP immunocytochemistry, Western blot and ELISA. Results In the process of stimulation by TGF - β1, the PAI- 1 staining counts of 0h, 12h, 24h and 48h group were 36.23 ±7.35, 62. 23 ±11.18, 98.58 ±4.98, 130.42 ±8.56 respectivley; and the PAI-1 protein level were 0.85 ±0.05, 1.21 ±0.06, 1.56 ± 0.08, 2.43 ± 0.02 respectively; and the PAI- 1 contents （ng/ml） in cell superants were 15.23 ± 1.98, 26.35 ± 2.93, 40.21 ± 1.54, 69.32 ± 3.21. Conclusions TGF -β, could induce PAI- 1 expression in RAW264.7 cells in a time - dependent manner.