摘要
建立了应用 RP—HPLC 法同时测定芦荟汁中芦荟苷和芦荟大黄素含量的方法.流动相:甲醇-水(55:45);检测波长:225nm;线性范围:芦荟苷为0.0162μg~0.162μg,r=0.9998,芦荟大黄素为0.0066μg~0.066μg,r=0.9997;平均回收率:芦荟苷为101.13%,RSD 1.39%,芦荟大黄素为100.05%,RSD 1.28%。该方法灵敏,准确,简便快速。
A new simultaneous determination for aloin and aloe-emodin in Aloe iuice by RP-- HPLC was established. The mobile phase consisted of methanol-water(55:45) and detection was at 225nm;the established method was linear in the range of 0.0162μg~0.162μg(r=-0.9998) for aloin and was linear in the range of 0.0066 μg~ 0.066 μg (r=-0.9997) for aloe-emodin;the average recovery was 101.13% with 1.39% of RSD for aloin and was 100.05% with 1.28% of RSD for aloin .The method was sensitive,simple and accurate.
出处
《生命科学仪器》
2006年第2期32-33,共2页
Life Science Instruments