栀子提取物ZG对单纯疱疹病毒1型细胞吸附的影响

Effect of the Extract ZG from Gardenia on the Cell Adsorption of Herpes Simplex Virus Type 1(HSV-1)

采用负染技术,借助高倍电子显微镜观察栀子提取物ZG作用后,病毒颗粒及其病毒吸附蛋白(virus attach-ment protein,VAP)的变化,考察药物是否直接改变或破坏病毒包膜蛋白的结构,使其失去感染性;采用异硫氰酸荧光素(fluorescein isothiocyanate,FITC)标记病毒,以肝素钠为参照,借助冷却慢扫描电荷耦合器件荧光成象技术,用Aquacomos软件进行图象分析,以探讨栀子提取物ZG不同加药方式对HSV-1吸附量的影响。结果表明栀子提取物ZG对HSV-1包膜表面的VAP无直接破坏作用,不影响病毒对Hep-2细胞的感染性;先加入肝素钠再进行病毒吸附及肝素钠病毒同时加入培养细胞这两种用药方式可明显减少细胞表面病毒的吸附量;栀子提取物ZG各种不同加药方式均能阻止HSV-1对Hep-2细胞表面的吸附,使病毒吸附量减少。

Negative-staining technique was used to investigate whether the extract ZG from Gardenia could change or destroy the virus envelope protein structure to weaken HSV-1 virulence. After treated with extract ZG from Gardenia, the HSV-I particles and the virus attachment proteins（VAPs） on virus surface were observed under electron microscope. Fluorescein isothiocyanate （FITC） as HSV-1 fluorescent probe and heparin sodium as the control samplc, we adopted the cooling charged-coupled device （cCCD） and Aquacomos analysis software to study thc effect of extract ZG from Gardenia on the cell adsorption quantity of HSV-1 in different methods. The results showed that extract ZG from Gardenia didn＇t directly destroy VAPs of HSV-1 . Both the group adding heparin sodium first and the group adding heparin sodium and virus simultaneously could prevent the viral bind ing to Hep 2 cells. Adding extract ZG from Gardenia in different ways all could prevent the HSV-1 binding to the Hep-2 cell surface and reduce the virus adsorption quantity.