重组人源抗狂犬病毒单克隆抗体SO57、SOJB对狂犬病毒G蛋白亲和力的比较研究

Affinity Comparison of Two Recombinant Human Anti-rabies Monoclonal Antibodies(SO57,SOJB) with Rabies Virus G Protein

It is desirable to develop potent rabies virus neutralizing human monoclonal antibodies(McAbs) to replace human or equine polyclonal immunoglobulin in the rabies virus postexposure treatment.Using BIAcore2000,affinity constants of McAbs with rabies virus glycoprotein(G protein) were determined taking human immunoglobulin as control,and affinity constants were calculated using BIAevaluation software.The affinity of McAb SOJB with rabies virus G protein was higher than that of SO57.Competition binding analyses ran on BIAcore2000 revealed that SO57 and SOJB competed for binding to rabies virus G protein,suggesting that epitopes recognized by SO57 and SOJB overlapped.Furthermore,the neutralizing potency of SO57 was significantly greater than that of SOJB as RFFIT showed.So it is desirable to develop SO57 firstly for rabies virus postexposure treatment.

It is desirable to develop potent rabies virus neutralizing human monoclonal antibodies（McAbs） to replace human or equine polyclonal immunoglobulin in the rabies virus postexposure treatment. Using BIA-core2000, affinity constants of MeAbs with rabies virus glyeoprotein（G protein） were determined taking human immunoglobulin as control, and affinity constants were calculated using BIAevaluation software. The affinity of MeAb SOJB with rabies virus G protein was higher than that of SO57. Competition binding analyses ran on BI-Acore2000 revealed that SO57 and SOJB competed for binding to rabies virus G protein, suggesting that epitopes recognized by SO57 and SOJB overlapped. Furthermore, the neutralizing potency of SO57 was significantly greater than that of SOJB as RFFIT showed. So it is desirable to develop SO57 firstly for rabies virus postexposure treatment.