华山松针叶表皮结构与抗疱锈病关系的初步研究

The Relation of the Blister Rust Resistance and the Structures of the Needle Epidermis in the Pinus armandii Franch

通过对来自云南4个华山松疱锈病典型发病区的122份华山松针叶样本的解剖结构研究,发现华山松的感病组与健康组之间在针叶表皮结构上有显著差异,其中主要差异为表皮细胞厚度和角质膜厚度,有抗病特性的植株具有显著加厚的针叶表皮结构,抗病的健康组比感病组的角质膜和表皮分别增厚达30.5%和8.0%,而气孔数量和内皮层厚度上差异不明显.结果支持疱锈菌感染华山松是通过主动侵入机制从表皮进入针叶,角质膜厚度和表皮厚度对华山松抵抗疱锈菌感染有重要作用.这一结果为弄清疱锈菌侵入华山松的途径提供了实验证据.

The anatomical structures of the needle leaves of Pinus armandii Franch were studied. All the 122 needle samples came from 4 typical blister-rust disease sites in yunnan Province. There was a significant difference in the structures of the needle epidermis between the infected samples and the healthy samples. The major difference existed on the thickness of the keratose and the epidermal cells. The structures of needle epidermis in the healthy samples have a thickening character; the thickening percentage is 30. 5% to keratose and 8. 0% to epidermal cells. The difference on the thickness of the endodermal structures and the stoma quantities between the two kinds of samples were insignificant. The results suggest that the rust fungi infected the Pinus arrnandii Franch via penetrating the needle foliages, and the structures of needle epidermis were a key defender to the infection of the rust fungi. This data provides an experimental proof to the rust fungi infection mechanism.