抗SmD1抗体测定对系统性红斑狼疮的诊断意义

Significance of anti-SmD1 antibody in diagnosis of systemic lupus erythematosus

目的:了解抗SmD1抗体对系统性红斑狼疮(system ic lupus erythem atosus,SLE)诊断的特异性与敏感性,探讨血清抗SmD1抗体水平与SLE病情的关联性。方法:将137例确诊为SLE患者和75例非SLE患者先采用线性印迹法定性检测SmD1。阳性者再采用ELASA法作定量分析,统计分析抗SmD1抗体对SLE诊断的特异性与敏感性以及与患者临床表现、实验室检查、病情活动性的关系。结果:线性印迹法检测抗SmD1抗体敏感性为84.9%,特异性为96.0%。抗SmD1抗体定量与系统性红斑狼疮病情活动积分呈正相关关系(P<0.01)。结论:线性印迹法检测抗SmD1抗体敏感性和特异性均较高,可作为诊断SLE的参考指标。抗SmD1抗体定量对判断SLE病情活动有一定的参考价值。

Objective：To investigate the sensitivity and specificity of anti-SmD1 antibody in diagnosis of systemic lupus erythematosus and the relationship between the level of antibody in sera and the activity of systemic lupus erythematosus （SLE）. Methods： The anti- StuD1 antibody in 137 cases with SLE and 75 cases without SLE were measured by line immuno assay（LIA）. The cases with positive results were determined by ELISA again. The specificity and sensitvity of anti-SmD1 antibody in sera of the patients were analyzed and the relations between the clinical manifestations, examination results and the active score of the patients were observed. Results：The sensitivity of anti-SmD1 antibody by LIA was 84.9% and the specificity was 96.0%. The level of anti-SmD1 antibody was positively related with SLEDMI（ r = 0.674 ,P 〈 0.01 ）. Conclusions ：The sensitivity by LIA is significantly higher than that by IBT. The level of anti-SmD1 antibody in sera is associated with the activity of SLE.