聚合酶链反应DNA扩增对活动性肺结核的诊断价值

DNA Amplification by Polymerase Chain Reaction for the Diagnosis of Active Pulmonary Tuberculosis

为探讨聚合酶链反应(PCR)DNA扩增对诊断活动性肺结核的临床价值,本文用PCR技术扩增结核杆菌特有的MPB64蛋白基因序列,对121例病人临床标本进行检测。发现34例活动性肺结核中有28例PCR阳性;疑诊为肺结核者33例中有18例阳性;非结核肺疾患54例中有10例阳性,其中有肺结核既往史者阳性6例,有结核接触史者阳性3例。提示PCR阳性并非为活动性肺结核所特有。

To evaluate the clinical application of the polymerase chain reaction(PCR)in the diagnosis of active pulmonary TB,the clinical samples from 121 patients were assayed by PCR to amplificate the gene encoding MPB64 protein,a DNA sequence unique to the Mycobacterium tuberculosis (M- TB)complex. The DNA of M. TB was identified in 28 of 34 patients with active pulmonary TB,and in 18 of 33 patients with suspected pulmonary TB. In 54 patients with other lung infections, PCR of 10 was positive. Six of the 10 patients had previously pulmonary TB,and 3 had contacted with TB patients. These data suggest that the positive PCR results are not unique for the patients with active pulmonary TB,and may be related to the infection of M. TB.