摘要
为提高血清学方法诊断幽门螺杆菌（Ｈｐ）感染的敏感性和特异性，建立了检测人血清中抗Ｈｐ抗体ＩｇＧ的免疫印迹方法。对因消化道症状而行胃镜检查的连续１３７例患者采集活检粘膜进行Ｈｐ培养、涂片和尿素酶试验，同时收集其静脉血，用免疫印迹方法检测血清中抗Ｈｐ抗体ＩｇＧ。１３７例患者中，１０４例患者胃粘膜用３种方法或其中２种方法检测Ｈｐ为阳性，３０例患者用上述３种方法检测Ｈｐ均为阴性，另３例患者仅１种方法检测Ｈｐ为阳性。Ｈｐ阳性患者的血清识别条带明显多于Ｈｐ阴性患者的血清。若以免疫印迹积分≥２分判为Ｈｐ阳性，＜２分为Ｈｐ阴性，则免疫印迹方法的敏感性为１００．０％，特异性为９０．０％，阳性预测值和阴性预测值分别为９７．２％和１００．０％。
SerologicaltestinghasrecentlybeenproposedasanaidindiagnosisofH.pyloriinfec-tions.Inthisstudy,animmunoblotmethodhasbeenevaluatedtodiagnoseH.pyloriinfectionserologi-calybycomparing104serumspecimensfrompatientswithapositiveGramstainand/orcultureresultandapositiveureasetestonbiopsymaterial,aswelas30serumspecimensfrompatientswithnegativeureasetest,andnegativemicroscopyandcultureresults.Thirteenbandscouldbeidentifiedbyim-munoblotingandwerefoundsignificantlymorefrequentinpatientswithH.pyloriinfectionsthaninpatientswithoutsuchinfections.Asemiquantitativegradingsystemwasintroducedwithscore1foreachreactivebandandscore1/2foraweaklyreactiveband.Ifscore2wasthecut-ofvalueforIgGan-tibody,thenthesensitivity,specificity,positivepredictivevalueandnegativepredictivevalueoftheim-munoblottestwere100.0%,90.0%,97.2%and100.0%respectively.Therewasnomarkedcrossre-actionwithbothCampylobacterjejuniandCampylobactercoli.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1996年第1期9-11,共3页
Chinese Journal of Internal Medicine
