促红细胞生成素对血管性痴呆大鼠认知功能的影响

Effect of Erythropoietin on Cognition of Vascular Dementia Rats

【目的】探讨促红细胞生成素(EPO)对血管性痴呆(VaD)大鼠认知功能的影响。【方法】采用永久性结扎双侧颈总动脉的方法建立VaD模型。将行为学筛选合格的Wistar大鼠分为假手术组(Sham)、VaD组、EPO侧脑室注射+VaD组(E1组)和EPO腹部皮下注射+VaD组(E2组)。应用Y-迷宫观察术后4周、8周、12周大鼠的空间学习和记忆能力。【结果】VaD组和治疗组(E1组和E2组)术后4周出现学习记忆能力降低,并呈逐渐下降趋势,VaD组为甚;而两治疗组之间无明显统计学差异。【结论】EPO能通过血脑屏障改善VaD大鼠的认知功能。

[Objective]To study the effect of erythropoietin（EPO） on cognition of vascular dementia（VaD）. [Methods]The bilateral common carotid arteries of Wistar rats were permanently ligated to established VaD models. The Wistar rats reaching standard were randomly divided into four groups： sham control group, VaD group, VaD＋ EPO lateral ventricle injection treatment（E1 ）group, and VaD＋ EPO abdominal hypodermic injection treatment（E2） group. The special study and memory in Y-maze after 4, 8, 12 weeks of operation were estimated. [Results]The rats in VaD group and treated groups （E1＋E2） showed worse performance 4 weeks after operation. A significantly timedependent decrease of performance could be measured in these groups. The rats in VaD group had more error numbers （EN） and longer total reation time （TRT） than treated groups at the same time; there was no significant difference existed between E1 group and E2 group. [Conclusion]Erythropoietin can cross the blood brain barrier （BBB） and improve the cognitive dysfunction of VaD rats.