表皮生长因子对腹膜间皮细胞增殖的影响

EFFECTSOFEPIDERMALGROWTHFACTOR(EGF)ONPROLIFERATIONOFHUMANPERI┐TONEALMESOTHELIALCGLLS

为探讨表皮生长因子（ＥＧＦ）对腹膜间皮细胞增殖的影响，以了解其在损伤腹膜修复中的作用及其机制，建立了人腹膜间皮细胞（ＨＭＣ）培养体系。采用真空负压ＡＢＣ免疫组化法分析了ＨＭＣ表面表皮生长因子受体（ＥＧＦ－Ｒ）的表达，并以３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入法对６例ＨＭＣ在不同培养时间和不同剂量ＥＧＦ作用下的细胞增殖程度进行了检测。结果表明，ＨＭＣ表面有ＥＧＦ－Ｒ存在，ＥＧＦ能促进ＨＭＣ增殖，当ＨＭＣ在含２０μｇ／ＬＥＧＦ的培养基中生长时，其３Ｈ－ＴｄＲ掺入量与对照组比较明显增加（Ｐ＜０．０１），而ＥＧＦ浓度５０μｇ／Ｌ时，ＨＭＣ增殖指数最大（ＰＩ＝８．１６），ＨＭＣ的增殖程度和ＥＧＦ呈时间与剂量依赖关系。提示，ＥＧＦ可能通过与ＨＭＣ表面的ＥＧＦ－Ｒ结合，促进细胞内的ＤＮＡ合成，加速细胞的增殖，对损伤腹膜的修复起重要作用。

INordertostudytheefectsofepidermalgrowthfactor(EGF)onproliferationofhumnperi-tonealmesothelialcels(HMC)andrepairrepairofdamagedhumanperitoneum,themesothelialcelsfromhumanomentumwereseperatedandculturedinvitro.TheauthorsassessedtheexpressionofEGF-RonthesurfaceofHMCwithABCimmunehistochemicalmethodandtestedtheproliferationex-tentofHMCtreatedwithEGFatdiferenttimeanddosagelevelswith3H-thymidineincorporationmethod.TheresultsshowedthatEGF-RexistedonthesurfaceofHMCandEGFcouldstimulatetheproliferationofHMC.TheproliferationofHMCgrowinginthemediacontaining20μg/LEGFwasmoresignificanthanthatgrowinginRPMI-1640alone.Thegreatestproliferationindex(PI=8.16)ofHMCappearedinthemediacontaining50μg/LEGF.TheproliferationofHMCstimulatedbyEGFwastime-anddose-depan-dent.ThesestudiesindicatethattheproliferationofHMCstimulatedbyEGFmayplayaveryimportantroleinthestructralandfunctionalrecoveryoftheinjuredperitoneum.