麻花秦艽组织培养及植株再生的研究

Studies on plantlet regeneration and propagation of Gentiana stramines

目的：研究药用植物麻花秦艽组织培养技术，为工厂化育苗提供科学依据。方法：以麻花秦艽根、茎育出的无菌苗为外植体，采用MS培养基，附加不同的植物激素进行实验。结果：以MS培养基为基本培养基，附加BA0．5～1．0mg·L^-1 ＋NAA0．5mg·L^-1，适于丛生芽的诱导与增殖；附加LAA 1．0mg·L^-1 ＋ BA3mg·L^-1，适于愈伤组织的诱导；附加姒1．0mg·L^-1 ＋BA2．0～3．0mg·L^-1适于愈伤组织继代培养，附加BA2．0mg·L^-1＋NAA0．5mg·L^-1适于愈伤组织的分化。结论：通过诱导愈伤组织途径可以达到快速繁殖的目的。

Objective： To provide scientific basis for large scale production by studying the technique of tissue culture of Gentiana stramines. Method： Callus was induced from germ-free stem segment of G. stramines on a MS medium supplemented with different hormones. Result： The MS medium with 0.5-1.0mg·L^-1 BA and 0.5 mg·L^-1 NAA was suitable for the induction and proliferation of cluster bads. MS medium with 1.0 mg·L^-1 IAA and 3 mg·L^-1 BA was suitable for the induction of calli. MS medium with 1.0 mg·L^-1 IAA and 2,0-3.0mg·L^-1 BA was suitable for the subculture of calli. MS medium with 2.0mg·L^-1 BA and 0.5mg·L^-1 NAA was suitable for the differentiation of calli. Condutton： Aseptic seeding of G. stramiess can be quickly propagated by shoot culture.