啤酒花中黄酮成分提取工艺的优化研究

Extraction optimization study of flavonoids from Humulus lupulus

目的:对啤酒花黄酮类化合物的提取工艺进行优化研究。方法:用单因素试验分别对提取溶剂乙醇含量、料液比、提取温度及提取时间进行研究,在此基础上通过正交试验确定上述4个因素的最佳组合。结果:优化后啤酒花中黄酮的最大提取量为78 mg.g-1。结论:啤酒花黄酮类化合物的适宜提取条件为乙醇含量45%、料液比1∶25、提取温度60℃、提取时间90 min。

Objective： To optimize extracting parameters of flavonoids from Humulus lupulus. Method： Based on the single factors test on ethanol concentration, material and solvent ratio, extracting temperature and extracting time, orthogonal test was performed and the best combination was confirmed. Result： With the optimized technology, the maximal extracting amount of flavonoids from H. lupulus was 78 mg·g^-1 .Condusion： the optimal techniques obtained are 45% ethanol extracting at 60℃ with material and solvent ratio 1：25 for 90 min.