固相萃取GC－FID和GC－MS快速测定血浆中中枢神经药物

Rapid determination of central nervous drugs in plasma by solid-phase extraction and GC-FID and GC-MS

目的：建立测定血浆中巴比妥类、安定类、吩噻嗪类和三环类等临床超剂量中毒病例中常见药物的方法。方法：用Ｘ－５树脂固相萃取分离纯化血浆中的药物，用毛细管气相色谱和ＧＣ－ＭＳ对药物进行定性和定量分析。结果：血浆中２０余种酸性、中性和碱性药物可同时被提取出来，并在毛细管气相色谱和ＧＣ－ＭＳ中得到很好的分离。线性方程的相关系数大于０．９９，大部分药物回收率的相对标准偏差（ＲＳＤ）小于１０％。在中性提取条件下，巴比妥类药物ＧＣ－ＦＩＤ的最低检出浓度为２～５μｇ／ｍｌ，在优化的提取条件下，可降至０．３～０．５μｇ／ｍｌ。结论：Ｘ－５树脂固相萃取的方法适合于血浆中大量药物的同时分离和纯化，结合毛细管气相色谱和ＧＣ－ＭＳ，可满足临床超剂量中毒分析的要求。

To establish a simultaneous determination method of central nervous drugs including barbitals,benzodiazepines,phenothiozines and tricyclic antidepressants in human plasma.METHODS:Drugs in plasma were extracted and purified by using X-5 resin solid phase extraction columns,followed by identification and quantitation using capillary GC-FID and GC-MS.RESULTS:More than 20 drugs were simultaneously extracted from human plasma,and effectively separated in GC and TIC spectra.The correlation coefficient of standard curves was larger than 0. 99,and relative standard differences(RSD)were less than 10% for most of the drugs.Under neutral extraction conditions,the lowest detection limits of barbitals were in the range of 2～5μg/ml,in optimized conditions,they were reduced to 0.3～0.5μg/ml.CONCLUSION:X-5 resin solid-phase extraction is fit for the simultaneous extraction and purification of large number of drugs in plasma,therefore it is satisfactory for rapid determination of overdose drugs clinicallly,when combined with capillary GC and GC-MS.