高效液相色谱法和分光光度法测定双氯芬酸钠乳膏含量的研究

HPLC and UV for concentration determination of diclofenac sodium cream

目的：研究和寻找精密而简便地测定双氯芬酸纳乳膏含量的方法。方法：采用高效液相色谱法和紫外分光光度法。结果：两种方法的平均回收率（ｘ％）及变异系数（ＲＳＤ％）分别为９９．４６±０．９ｌ和９９．７０±０．９２。结论：结果表明，两种方法均可用于双氯芬酸钠乳膏的含量测定，但ＨＰＬＣ更适于成品含量测定和杂质检查。而ＵＶ法可用于生产过程中的半成品检验。

To find a precise,simple and convenient method for concentration determination of diclofenac sodium cream. METHODS :HPLC and UV were used to determine its concentration. RESULTS:Analytical recoveries of HPLC and UV were 99.46%and 99.70%respectively. The RSD was 0.9l%of HPLC and 0.92%for UV.CONCLUSION:The results show that the former can be used to determine finished product and impurity check up and the latter to determine sime-finished product.