肺癌中EB病毒的原位杂交检测

Detection of EB virus in pulmonary carcinoma by in situ hybridization

为观察EB病毒在肺癌组织中的分布并探讨EB病毒感染与肺癌的关系，对87例肺癌组织中经多聚酶链反应（PCR）检测52例EB病毒阳性的石蜡包埋组织，用原位杂交技术进行定位检测。癌组织及癌旁肺组织原位杂交EB病毒阳性率分别为37．9％（33／87）和11．5％（IO／87）；中、低分化癌与高分化癌EB病毒阳性率有显著差异，强阳性病例均为分化较差的肿瘤；累及胸膜、肋骨、隔肌的肺癌，其EB病毒阳性率明显高于未侵及者；随着EB病毒感染的加重，淋巴细胞浸润增多。结果表明：EB病毒感染与肺癌细胞的生物学特性及肺癌的发展有关。

To bserve the distribution f Epstein-Barr virus (EBV) in pulmonary carcinoma, 87cases of pulmonary carcinoma were used to detect EBV infection by in situ hybridization. The psitive rate of EBV infection in carcinoma tissue and peripheral lung tissue were 37.9% (33/87) and 11. 5 %(10/87) respectively. The existence of EBV was related to both the degree of carcinoma differentiation and lymphocyte infiltration. The EBV positive rates and hybridization signals in the groups of moderately and poorly differentiated carcinma were significantly higher and stronger than those in the group of well differentiated carcinoma. The degree of lymphocyte infiltration increased with the increase of EBV positive rate. These results indicate that there is a relationship between EBV infection and the biological features of cancer, and that EBV may p]ay a role in the pathogenesis of pulmonary carcinoma.