红根草的组织培养与快速繁殖研究

Tissue culture and rapid propagation of Salvia prionitis

研究了红根草不同采集季节、外植体类型与消毒效果，不同激素浓度和激素组合与芽及根的诱导，及试管苗的移栽。结果表明，不同外植体类型消毒效果为：茎节〉茎尖〉植株抽茎前的生长点14～6月份采样最佳；MS＋BA0．8～1．6mg／L＋NAA0．2mg／L、MS＋BA0．2mg／L＋NAA0．02mg／L、MS＋NAA1.0mg／L分别用于初代、继代、生根培养效果最好；试管苗移栽成活率达90％。

Tissue culture and rapid propagation of Salvia prionitis were reported in this paper. Effects of several factors such as different explants, collecting periods on the influence of sterilization, different phytohormones concentration,medium on the induction of buds and roots, and transplantation of seedlings were studied. Results showed that stem was the best explant for sterilization,the next one was the tip of stem and the third one was the low tip on the ground before the stem outgrowth. The best collecting periods of explants was from April to June. The most suitable medium for bud induction was MS＋BA 0.8-1.6 mg/L＋NAA 0.2 mg/L,MS＋BA 0.2 mg/L＋NAA 0. 02 mg/L was optimum for proliferation, and MS＋NAA1.0 mg/L was best for root induction. The survival rate of the seedlings was about 90%.