532-二极管激光光凝法诱导大鼠青光眼慢性高眼压模型的研究

Establishment of the rat model of chronic glaucoma by laser photocoagulation of semiconductor frequency-doubled 532 laser argon laser

目的:建立532—二极管激光光凝的大鼠慢性高眼压性青光眼模型,评价模型眼的相关组织病理学改变。方法:选用成年SD大鼠50只,采用532-二极管激光行角膜缘小梁网和角膜缘颞侧及颞上、颞下3条巩膜浅层静脉光凝。定期测量和记录双眼眼压,观察眼前节情况。于术后1、3,6个月时摘取双侧眼球,观察双眼视网膜组织形态学改变、视网膜厚度及视网膜神经节细胞数目的改变。结果:(1)大鼠眼内压于1个月时达到眼压峰值,高眼压状态持续2月余,然后眼压水平逐渐下降,6个月时眼压基本恢复到正常范围;(2)视网膜厚度明显变薄,视网膜神经节细胞数明显减少。结论:532-二极管激光光凝SD大鼠小梁网及巩膜浅层静脉血管可以建立长期眼压中度升高的慢性高眼压性青光眼模型,模型眼出现了青光眼眼底特征性的形态学改变。

Objective To establish a chronic glaucoma model in SD rats and to evaluate the related pathological changes. Methods：Laser photocoagulation was applied to 50 adult SD rats by semiconductor frequency - doubled 532 laser argon laser. The laser spot was aimed at the trabecular meshwork and episcleral veins. Tono - pen XL Tonometer was used to measure the intraocular pressure（lOP） to gurantee lOP value at 3 day, 1 week, 1,2 3, 6 month in both model and control eyes. Eyes of experimental rat were enucleated in 1, 3 and 6 months after operation to observe the changes of retina, optic nerve and numbers of retina ganglion cells of central and peripheral retina to judge whether the model was successful. Results： After the laser treatment,42 rats model with intraoular hypertension were obtained. The angle of anterior chamber were narrow, and the fibroblasts aggregation in trabeeular retieulum, the iris were also abnormal obviously. Retina thickness became thinner with obviously loss of retina ganglion cells in the eentral and peripheral retina, Conclusion; Establishment of chronic glaneoma model by unilaterally photoeoagulating the trabeeular meshwork and episcleral veins in SD rats offers a reproducible and quantitative way for glancomatous study. Compared with the control eyes, the modd eyes present characteristic glaueomatous morphological changes.