皮肤鳞状上皮细胞肿瘤中p53蛋白的表达

Study of P53 in Cutaneous Squamous Epithelial Cell Tumour

基因突变在肿瘤的发病机制中起着重要作用。p53基因有2种类型,野生型和突变型。为了探讨 p53基因突变与皮肤鳞状上皮细胞肿瘤发生、发展的关系,本研究采用一种新的免疫组化技术标记的链霉亲和素-生物素连接系统对29例皮肤鳞状上皮细胞肿瘤皮损进行免疫组化研究。结果3/6例日光性角化 p53染色阳性,p53阳性细胞局限于表皮基底部位非典型细胞增生区域。6/10例 Bowen 病和8/13例鳞状细胞癌 p53染色阳性,p53阳性细胞弥漫分布于肿瘤组织全层。提示 p53基因突变在皮肤鳞状上皮细胞肿瘤的发生、发展中起着重要作用。

Gene mutation is known to play an important role in carcinogenesis.There are two types of p53 gene,wild-type and mutant-type.In order to observe the relationship between p53 gene mutation and cutaneous squarnous epithelial ceil tumours,immunohistochemical study was carried out for p53 ex- pression in the epidermis from 29 patients with cutaneous squamous epithelial cell tumouts using a new immunohistochemical technique labelled streptavidin biotin system.The results showed that p53 positive staining was only confined to areas of atypical proliferation of epithelia in 3/6 cases of actinic keratosis. 6/10 cases of Bowen's disease and 8/13 cases of squamous cell carcinoma showed that p53 positive staining was seen throughout the tumour.It is suggested that p53 gene mutation might play an impor- tant role in cutaneous squamous epithlial cell tumour formation.