TGF-β1、CD105、CD34在胰腺癌中的表达及其临床意义

Expressions and clinical significance of TGF-β1,CD105 and CD34 in pancreatic carcinoma

目的:探讨TGF-β1、CD105、CD34在胰腺癌中的表达及临床意义。方法:应用免疫组化SP法检测三者在52例胰腺癌和10例非肿瘤性胰腺组织中的表达,并分析它们与胰腺癌临床病理参数及患者生存率间的关系。结果:TGF-β1在非肿瘤性胰腺组织、胰腺癌中的阳性表达率分别为30%、80.77%,两者间的差异具有统计学意义(P<0.01)。胰腺癌中,CD105、CD34标志的微血管密度(MVD)均明显高于非肿瘤性胰腺组织。TGF-β1、CD105、CD34的表达水平与胰腺癌临床分期及淋巴结有无转移呈正相关,且CD105与胰腺癌的病理分级有关。TGF-β1与CD105、TGF-β1与CD34在胰腺癌中均有协同表达,CD105在胰腺癌中的表达较CD34特异性高。此外,生存单因素分析TGF-β1、CD105、CD34均与胰腺癌的预后有关。结论:TGF-β1、CD105、CD34在胰腺癌生长、浸润和转移过程中发挥了一定作用。CD105较CD34能更准确的反映内皮细胞的增殖状况。

Objective：To study the expression and clinical significances of TGF-β1,CD105 and CD34 in pancreatic carcinoma. Methods：The expressions of the three proteins were detected by SP immunohistochemical method in 52 cases with pancreatic carcinoma and 10 cases of non tumorous pancreatic tissue. Their relationships with clinic-pathological features and survival rate were also analyzed. Results： The positive ratio of TGF-β1 expression in non tumorous pancreatic tissue and pancreatic carcinoma was 30.00% and 80.77%, respectively. The difference was significant （P〈0.01）. The microvessel density （MVD） marked by CD105 or CD34 in pancreatic carcinoma was markedly higher than that in non tumorous pancreatic tissue. The expression level of TGF-β1 ,CD105, and CD34 was positively correlated with clinical stages and metastasis of lymph nodes. There was co-expression of TGF-β1 and CD105 or TGF-β1 and CD34 in pancreatic carcinoma. The expression of CD105 had higher specificity in pancreatic carcinoma than CD34. The single-factor analysis revealed that the expression of TGF-β1,CD105,and CD34 was significantly correlated with the prognosis of pancreatic carcinoma. Conclusion： TGF-β1 ,CD105,and CD34 played an important role in growth,invasion and me tastasis of pancreatic carcinoma. CD105 was more specific than CD34 to reflect the proliferation of endothelial cells.