温热与羟基喜树碱（OPT）联合对胃腺癌（SGC－7901）细胞的作用

Effect of Combined Use of Hyperthermia and HYD Oxy-camptothecia(OPT)on Human Stomach Low Differentiation Cancer Cell Line SGC-7901 in vitro

以体外长期培养胃低分化腺癌细胞系（ＳＧＣ－７９０１）细胞为模型，采用水浴加温法对细胞的生长抑制规律进行了观察。表明：１）温热、ＯＰＴ对ＳＧＣ－７９０１细胞均有一定的抑制增殖和直接杀伤作用；温热与药物联合应用具有协同抑制增殖和杀伤细胞的作用。２）温热和ＯＰＴ联合对ＳＧＣ７９０１细胞的杀伤和生长抑制作用均较温热或药物单独作用强，但序贯方面以热药同时为佳。３）温热、ＯＰＴ都可使ＳＧＣ－７９０１细胞结构发生变化，单用以细胞变性为主，联合组多为不可逆性损伤。４）温热４０℃作用后细胞爆发性生长，表明单纯加热低于４２℃是不适宜的。

The low differentiation stomach gland cancer cell line(SGC-7901) which was established by Shanghai Sixth People's Hospital was used in this study.The inhibition pattern of cell growth was observed under water-heating with or without OPT(Camptothecia derivative).The results showed that:1.Both hyperthermia and OPT can inhibit the proliferation of the SGC-7901 cells and kill the cells directly.The inhibition effect can be enhanced by adding hyperthermia. 2.Hyperthermia in combination with OPT can check proliferation or kill more cells than singly hyperthermia or chemical agent. But the best killing effects of the sequences can be achieved when heating and the chemical drug used simultaneously.3.Both hyperthermia and OPT can change the cell structure.The principal change observed was cell degeneration when these factors were given alone, however, it was unreversible damage when combined.4. When heated at 40℃ for an hour, the cells growth kindled tremendously indicating that temperature lower than 42℃ is unfavorable in tissue of therapy.