IL-2、IL-4和IL-7体外诱导人外周血淋巴因子激活的杀伤细胞效应的比较

EVALUATION OF THE EFFECTS OF IL-2, IL-4 AND IL-7 ON THE INDUCTION OF HUMAN LYMPHOKINE-ACTIVATED KILLER CELLS

比较了淋巴因子ＩＬ－２、ＩＬ－４和ＩＬ－７在体外诱导健康人外周血淋巴因子激活的杀伤 （ＬＡＫ）细胞活性的效应。结果表明，ＩＬ－７可单独，亦可与ＩＬ－２、ＩＬ－４协同诱导ＬＡＫ细胞，而且ＩＬ－７ 诱导ＬＡＫ细胞的效应不被抗ＩＬ－２、抗ＩＬ－４所抑制。ＩＬ－７单独诱导的ＬＡＫ细胞活性高峰迟于ＩＬ－ ２或ＩＬ－４所诱导的活性高峰，且与增殖反应曲线一致。抗ＣＤ８抗体明显抑制ＩＬ－７诱导ＬＡＫ细胞 的效应，而ＩＬ－７诱导ＬＡＫ细胞的效应不能被抗ＮＫＨ－１所抑制。提示：ＩＬ－７ 激活ＬＡＫ细胞的效应 机制不依赖ＩＬ－２和ＩＬ－４，并很可能成为肿瘤过继治疗中的重要淋巴因子。

Had investigated and compared the effect of IL-2, IL-4 and IL-7 on the generation of lymphokine-activated killer (LAK) cell activity in human peripheral blood mononuclear cells of healthy individuals. The data demonstrated that the kinetics of IL-7 induced LAK activity peaked at day 8 while that of IL-2 or IL-4 induced LAK activity peaked at day 4. In lymphokine activated proliferative response a similar pattern of responsiveness was obtained. A synergy was found and maintained over longer periods of time when IL-7 was added together with IL-2 or IL-4. However,when we studied the effects of anti-IL-2 or anti-IL-4 on IL-7 induced LAK activity,we found that neither IL-2 nor IL-4 might influence the generation of LAK cells mediated by IL-7. The function analysis of CD8, CD4 and NKH-1 on lymphokine mediated LAK activiy indicated that LAK activity induced by IL-7 was completely eliminated by treatment with anti-CD8,but not by anti-CD4 or anti-NKH-1. These results suggest that IL-7 may act independently of IL-2 and IL-4 and is more effective on the induction of human LAK cells, either alone or in combination with IL-2 or IL-4, could be very important for adoptive immunotherapy.