我国藏族人群补体第四成分单倍型的分布分析

DISTRIBUTION AND ANALYSIS OF THE FOURTH COMPONENT OF C4 HAPLOTYPE IN THE CHINESE TIBETAN

采用武汉同济医科大学中澳友谊补体实验室仿国际标准Ｃ４定型程序改进后建立的 方法，对９９名无血缘关系的藏族人进行补体Ｃ４单倍型检测。对１９８个Ｃ４单倍型检测结果分析， 我国藏族人群Ｃ４单倍型频率以Ａ３Ｂ１最高（０．４１９２），Ａ３Ｂ２次之（０．１８６８），以下依次是：Ａ４Ｂ２ （０．０７０７）、Ａ２Ｂ１（０．０７０７）、Ａ３Ｂ５（０．０４５４）等。在Ｃ４Ａ座位上发现８个型别：Ｃ４Ａ５、４、３、２、１、３４、４５ 及 Ｑ０，在Ｃ４Ｂ座位上发现 ９个型别：Ｃ４Ｂ５、１２、２、１、４、９６、２６、２． １及Ｑ０。分类归属 ２４种，共组成 ３４ 种不同表型。将Ｃ４表型的观察值与预期应进行比较，卡方检验发现Ｃ４Ａ差异具有极显著意义 （Ｃ４Ａ，ｘ２＝３４．６７９８，Ｐ＜０．００５），而Ｃ４Ｂ没有统计学意义（Ｃ４Ｂ，ｘ２＝８．９１００，Ｐ＞０．５）．Ｃ４Ｂ在人 群分布符合Ｈａｒｄｙ－Ｗｅｉｎｂｕｒｇ（Ｈ－Ｗ）平衡。

With the method improved by Sino-Australian Friendship Complement Laboratory of Tongji Medical University in Wuhan according to C4 definite process of international standard,we examined C4 haplotypes in 99 no-sib Tibetans. The result of C4 haplotypes in 198 cases showed the frequencies as A3B1 (0. 4192 ), A3B2 (0. 1868), A4B2 (0. 0707), A2B1 (0. 0707),A3B5 (0. 0454). The 8 types are discovered at C4A and named as C4A5, 4, 3,2, 1, 34, 45 and Q0 respectively. The other 9 types are discovered at C4B and named as C4B5, 12, 2, 1, 4, 96, 26, 2. 1 and Q0. Compared the observed value of C4 phenotypes with the expected value, the difference of C4A in x2 test show great significance (C4A, x2= 34. 6798, P <0. 005),while C4B is no statistic value (C4B, x2=8. 9100, p > 0. 5). The distribution of C4B in Tibetan population coinsides with Hardy-Weinburg Law.