长期HBV携带者IFN-γ的诱生和TNF-α的检测

MEASUREMENT OF INDUCED INTERFERON-γ AND TUMOR NECROSIS FACTOR-α OF LONG-TERM HEPATITIS B VIRUS (HBV) CARRIERS

应用免疫放射量度分析和ＥＬＩＳＡ双抗体夹心法分别检测了６８ 例长期ＨＢＶ携带者 ＰＢＭＣ诱生ＴＮＦ－γ和ＴＮＦ－α的含量．结果表明：①ＨＢＶ无症状携带者和肝炎携带者诱生ＩＦＮ－γ 活性显著低于正常对照（Ｐ＜ ０． ０５或 ０． ０１），②肝炎携带者血清和培养上清中 ＴＮＦ－α的含量均高 于正常对照和无症状携带者，而无症状携带者诱生ＴＮＦ－α的含量又比对照组低；③各组的ＩＦＮ－γ 和 ＴＮＦ－α之间相关性检验无显著意义。提示：长期ＨＢＶ携带者诱生 ＩＦＮ－γ和 ＴＮＦ－α的能力异常， 可能是导致ＨＢＶ长期携带原因之一。

Interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) produced by peripheral blood mononuclear cells (PBMC) from 68 cases of long-term hepatitis B virus (HBV) carriers were measured respectively with immunoradicometric assay and double-antibody sandwich ELISA. The results showed that: (1) the activity of IFN-γ from asymptomatic HBV carriers and chronic HBV infection was obviously lower than that from normal controls (P <0. 05 or 0. 01) ; (2) TNF-α levels of in serum and cultured supernatants by PBMC from chronic HBV infections were increased compared with normal controls and asymptomatic HBV carriers, but TNF-α levels of asymptomatic HBV carriers was significantly lower than that normal control; (3) correaction between IFN-γ and TNF-α from every groups was not significant. The results indicated that capibility of IFN-γ and TNF-α production were abnormal, which may be one of causes leading to long-term carring HBV.