维甲酸与二甲基亚砜诱导HL－60细胞及其抗性亚型MDR　1的表达和对Rhodamine－123外排的影响

INDUCTION OF EXPRESSION OF MDR 1 GENE BY RETINOIC ACID AND DMSO AND EFFECTS ON RHODAMINE-123 EFFLUX IN HL-60 CELL LINES AND RESISTANT SUBLINES

利用Ｄｏｔｂｌｏｔ和流式细胞术，研究了分化诱导剂维甲酸，ＤＭＳＯ对ＨＬ－６０细胞及其抗性亚型抗药性程度的影响，表明１μｍｏｌ·ｍｌ^(－１)ＲＡ作用ＨＬ－６０及其亚型２４ｈ，ＭＤＲ１ｍＲＮＡ明显增高，但流式检测多药抗性细胞系对Ｒｈｏ－１２３的外排有所下降。２％ＤＭＳＯ作用ＨＬ－６０及其亚型２４ｈ，流式细胞术检测显示各细胞系对Ｒｈｏ－１２３的外排明显增强，提示ＲＡ虽然提高ＭＤＲ１基因的表达，但可能通过磷酸化／脱磷酸化方式抑制Ｐｇｐ－１７０功能的表达，而ＤＭＳＯ能诱导完整功能的Ｐｇｐ表达。

Using dot blot hybridization and flowcytometry,the effects of differentiationinducers retinoic acid(RA)and dimethvl sulfoxide(DMSO)on the resistant level of HL-60 cells andits resistant subline cells were studied.When the cells were treated with RA 1 umol·L-1 for 24h,the expression of MDR 1 mRNA evidently increased in both HL-60 and its multidrug resistant sublinecells.The efflux of Rho-123 in the multidrug resistant subline cells was slightly decreased,But,whenthe cells were treated with 2%DMSO for 24 h the efflux of Rho-123 increased obviously.The resultssuggest that RA can induce the expression of MDR 1 gene but perhaps inhibit the function of pumpglycoprotein 170(Pgp-170)through phosphorylation/dephOsphorylation pathway.However,DMSOcould induce the expression of full function of Pgp。