人体抑制素α亚基片段的合成及活性研究

SYNTHESIS AND ACTIVITIES OF FRAGMENTS OF INHIBIN α SUBUNIT

用固相多肽合成法的Ｆｍｏｃ化学合成了４个人抑制素α亚基片段，经三氟醋酸裂解、高效液相纯化，产物总收率２０％～６３％。考察人工合成的四种抑制素α亚基肽片段对大鼠离体培养黄体细胞孕酮分泌的影响，结果表明４种抑制素α亚基肽片段均显著抑制大鼠黄体细胞的基础孕酮分泌。

For further investigation of inhibin which is available only by laborious isolation,four fragments of inhibin α subunit(7-28,37-65,1-32,Y-1-32)were prepared by solid phasepeptide synthesis using the Fmoc strategy.The effects of these fragments on progesterone production ofrat corpus luteal(CL)cells in vitro were examined.The results indicate that these four fragmentsinduced significant decrease of basic progesterone production.