微流动注射芯片化学发光法检测鱼虾中的四环素

Determination of Tetracycline in Fish and Shrimp by Chemiluminescence Micro-flow Injection on a Chip

在微流动注射芯片上设计了螺旋形流通池，并采用精确时间控制进样方式，结合四环素（TC）对鲁米诺-H2O2化学发光体系的抑制作用，建立了微流动注射芯片化学发光法测定鱼虾中四环素的新方法。该系统中螺旋形反应池由激光雕刻机在聚甲基丙烯酸甲酯（PMMA，50×40×3mm）上雕刻而成。在实验所建立的最佳条件下，该方法在2．0×10^-7～1．0×10^-5g／mL范围内化学发光的降低值与四环素的质量浓度具有好的线性关系．检出限为1．7×10^-8g／mL。相对标准偏差为1．7％（n=11，p=5．0×10^-7g/mL），检测结果满意。

A micro-flow injection system on a chip was prepared for the determination of tetracycline in fish and shrimp, based on luminol- H2O2 chemilunlineseence. Spiral flow cell channels were fabricated using laser on poly（ methyl methacrylate） （PMMA）. The sampling time was controlled precisely by atimer, The linear range was 2.0×10^-7～1.0×10^-5g/mL with a detection limit of 1.7×10^-8g g/mL, and the relative standard deviation was 1.7% （ n =11, p = 5.0 ×10^-7 g/mL）, The method is simple, rapid and highly sensitive and consumes less reagent, The proposed method has been successfully applied to the determination of tetracycline in fish and shrimp,