麦麸酶解产物对糖尿病大鼠抗氧化能力的影响

Effect of enzymes-hydrolyzed wheat bran on antioxidant ability in diabetes rats

目的:观察麦麸酶解产物对糖尿病大鼠抗氧化系统的影响。方法:用四氧嘧啶制备SD大鼠糖尿病模型,以麦麸酶解产物(EHWB)为受试物,阿魏酸钠作为阳性对照,测定各实验组大鼠血糖、血清和肝脏总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和黄嘌呤氧化酶(XOD)活性及丙二醛(MDA)含量。结果:与糖尿病组相比,EHWB治疗组血糖明显降低(P<0.01),血清和肝脏T-AOC均提高(P<0.05),GSH-Px和SOD活性增强(P<0.05),XOD活性及MDA含量明显降低(P<0.05),各项指标与强抗氧化药物阿魏酸钠比较差异无显著性(P>0.05)。结论:EHWB具有降血糖和提高糖尿病大鼠抗氧化能力的作用,其效果与阿魏酸钠相似。EHWB的降糖作用可能与其恢复胰腺功能,保护肝脏的抗氧化作用有关。

Objective To observe the effect of enzymes-hydrolyzed wheat bran （EHWB） on antioxidant system in diabetic rats. Methods Diabetes mellitus models were indueed by alloxan injection and EHWB was used to test its effect on anti-oxidative capacity; the EHWB was given intragastrieally （i. g. ）, sodium ferulate （SF） as a positive group. The level of blood glucose, total anti-oxidative capacity （T-AOC）, the aetivities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, and xanthine oxidase （XOD）, and the content of malondiadehyde （MDA） were detected respeetive}y in serum and liver of rats. Results EHWB significantly decreased the level of blood glucose and increased the level of T-AOC, as well as the activities of GSH-Px and SOD, and decreased the activity of XOD and the content of MDA. However, there was no difference between the groups feeding SF and EHWB. Oonclusion EHWB ean he used to decrease blood glucose and improve antioxidant ability in diabetes mellitus rats, the effect of EHWB is similar to SF. The effeet of decreasing blood glucose of EHWB is related to protecting liver.