白芍总苷对犬急性心肌缺血的保护作用

Protective effects of total glycosides of Pacony on acute myocardial ischemia in dogs

目的:研究白芍总苷(TGP)对犬急性心肌缺血的保护作用。方法:取杂种犬30只,随机分为5组(每组6只),分别为模型对照组、阳性对照药物(复方丹参注射液)组及TGP 2.0、4.0和8.0 mg.kg-1剂量组;采用结扎麻醉开胸犬左冠状动脉前降支(LAD)制备急性心肌梗塞模型,观察TGP对心外膜电图(EECG)、心肌梗塞面积(MIS)和血清心肌酶的影响。结果:TGP各剂量组与模型对照组比较,心肌缺血程度(Σ-ST)减轻(P<0.05,P<0.01),心肌缺血范围(N-ST)缩小(P<0.05),MIS缩小(P<0.05,P<0.01),血清中磷酸肌酸激酶(CPK)和乳酸脱氢酶(LDH)的活性降低(P<0.05,P<0.01),游离脂肪酸(FFA)和过氧化脂质(LPO)含量降低(P<0.05,P<0.01),超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性提高(P<0.05,P<0.01)。结论:TGP对实验性缺血心肌具有保护作用。

Objective To study the protective effects of total glycosides of Pacony （TGP） on acute myocardial infarction in dogs. Methods Thirty domestic dogs were randomly divided into 5 groups （6 in each group ） ： model control group, positive control group （ISM）, and TGP groups with doses of 2.0, 4.0, and 8.0 mg·kg^-1, respectively. The descending branch of left coronary artery was ligated to construct the model of myocardial infarction in the anesthetic thoraeo-opened dogs. The effect of TGP on epicardium electrocardiogram （EECG） , myocardial infarction size （MIS） and serum myocardial enzymes were observed. Results Compared with controls, the ischemia degree （∑-ST, P〈0.05, P〈0.01）, ischemia area （N-ST, P〈0.05） and infarction area were reduced in TGP groups with various doses, the activities of serum creatine phosphoric kinase （CPK） and lactate dahydrogenase （LDH） were deceased （P〈0.05, P〈0.01）. Moreover, the levels of free fatty acids （FFA） and lipoperoxdation （LPO） were decreased （pcaos, pcaol）, the activities of serum superoxide dismutase （SOD） and glytathion-peroxdation （GSH-Px） were increased （P〈0.05, P〈0.01 ）. Oonclusion TGP has effects on protection of the ischemic myocardial tissue.