乳痛宁对大鼠乳腺增生病模型的作用

Effects of Rutongning on hyperplasia of mammary glands in rats

目的:探讨乳痛宁对大鼠乳腺增生病模型的作用效果和机理。方法:Wistar雌性未孕大鼠50只,随机分为正常对照组、模型组及低、中、高剂量乳痛宁组,每组10只,用雌二醇和黄体酮诱导大鼠发生乳腺增生病,用乳痛宁进行灌胃,测量大鼠第2对乳头直径,观察乳腺组织结构,测定血液中生殖激素水平,免疫组化法测定乳腺组织中雌、孕激素受体阳性细胞。结果:中、高剂量乳痛宁组与模型组大鼠比较血液中的催乳素、促卵泡激素和雌二醇的浓度明显降低(P<0.05),黄体生成素和孕酮则明显升高(P<0.05),大鼠第2对乳头直径明显减小(P<0.05),腺泡和腺管数目减少,体积缩小(P<0.01),雌、孕激素受体阳性细胞明显减少。结论:乳痛宁通过调整大鼠体内的激素分泌水平,减弱雌激素和孕激素在乳腺的生物学效应,从而使增生的乳腺恢复正常。

Objective To discuss the effects and mechanism of Rutongning capsule on hyperplasia of mammary glands in rats. Methods 50 female Wistar rats without pregnancy were randomly divided into normal group, model group, low dosage group, middle dosage group and high dosage group （n=10）. Estradiol and progesterone were used to induce hyperplasia models of mammary glands in rats. The rats with hyperplasia of mammary glands were administered by gavage with Rutongning. The diameter of the second twin nipples of the rats was measured and the structure of the mammary gland tissue was observed. The level of hormone in blood was detected by radioimmunity and positive cells of estrogen receptors （ER） and progesterone receptors （PR） were determined by immunohistochemical technique. Results Compared with model group, estradiol, prolactin, follicle stimulating hormone in middle and high dosage groups were decreased （P〈0.05）, and luteinizing hormone , progesterone were increased （P〈0.05） , and the diameter of the second twin nipples of the rats was reduced （P〈0.05）. Rutongning made acini atrophy and make gland ducts, and their volumes became small significantly （P〈0.01）. Rutongning decreased the number of ER and PR positive cells significantly. Conclusion Rutongning could rectify the secretion of hormone in rats and weaken the biological effects of estrogen and progesterone on mammary glands, so the hyperplasia mammary glands could get recovery.