大肠癌多药耐药细胞蛋白激酶C的表达及作用

Expression of protein kinase C in multidrug resistant cells of colorectal carcinoma

目的:检测电离辐射作用后大肠癌多药耐药细胞蛋白激酶C(PKC)的表达和观察蛋白激酶C在多药耐药发生发展中的作用。方法:对大肠癌HCG-8多药耐药细胞照射,照射后用间接免疫荧光技术,采用流式细胞仪检测X射线对HCG-8细胞PKC表达的影响。结果:与假照组相比,2 Gy大剂量照射后PKC阳性细胞百分率明显增加(P<0.05),先给予低剂量照射(50和100 mGy),再给予大剂量照射,PKC阳性细胞百分率增加不明显,200 mGy+2 Gy组PKC阳性细胞百分率明显增加(P<0.05)。与单纯2 Gy大剂量照射组比较,100 mGy+2 Gy组PKC阳性细胞百分率明显降低(P<0.05)。结论:大剂量辐射可使大肠癌细胞PKC蛋白表达明显增加,而预先给予低剂量辐射后再给予大剂量辐射可使大剂量辐射增强PKC蛋白表达作用不明显。

Objective To detect the expression of protein carcinoma after ionizing irradiation and observe the effect kinase C （PKC） in multidrug resistant cells of coloreetal of PKC on occurrence and development of the multidrug resistant. Methods The effect of PKC on multidrug resistant HCCr-8 cells of colorectal carcinoma after treated with X-ray was detected by indirect immunofluorescence technique and flow cytometry. Results Compared with shamirradiation group, the positive percentage of PKC was increased significantly （P〈0.05） after HCT-8 cells were treated with 2 Gy X ray, the positive percentages of PKC were increased insignificantly after HCT-8 cells were treated with low dose （50, 100 mGy） before high dose of irradiation, the increasing of positive percentage of PKC in the group of 200 mGy＋2 Gy was significant （P〈0.05）. Compared with the group only treated with high dose of irradiation, the positive percentage of PKC in the group of 100 mGy＋2Gy was decreased significantly （P〈0.05）. Conclusion High dose of ionizing irradiation can increase the expression of PKC in coloreetal carcinoma significantly, reversely when HCT 8 cells are pretreated with low dose before high dose there is no additional increase of PKC expression.