大豆体细胞胚再生植株的研究

A STUDY OF SOYBEAN PLANT REGENERATION VIA SOMATIC TISSUE CULTURES

以球形期大豆体细胞胚为材料,对其进行继代增殖、萌发及再生植株的研究。结果表明,大豆体细胞胚每隔15-20天分割成3mm左右的小块在MS培养基附加10-20 mg/L 2,4-D的固体培养基以及弱光条件下,可以继续增殖,继代增殖能力强,扩繁系数为3n(n为继代培养次数);大豆体细胞胚性组织先在含有活性碳的培养基上培养,能提高其萌发率以及正常胚率,1个月以后转移到无活性碳的培养基,又能提高其再生速度以及再生率。

The system of tissue culture were studied about sparal somatic embryos of soybean,the media about the subculture,germination and regeneration were established. The result showed that the media for somatic embryogenesis proliferation was MS with 10-20mg/L 2,4-D and weak light when they were divided into part somatic embryos with dismater 3mm every 15-20 day. The subculture coefficiency reached 3^n, （ ＂n＂was the times of proliferation culture ）, the germination frequency and normal somatic embryos frequency of soybean increased when active carbon was applied; when they were cultured one month under this condition, again they were transferred into the media without active carbon, the regeneration frequency and the regeneration speed of soybean increased.