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浓度梯度凝胶配制的简易装置设计及应用效果 被引量：1

DESIGN SIMPLE EQUIPMENT FOR MAKING DENATURE GRADIENT GEL
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摘要 PCR-DGGE是分子生物学工作者分析微生物区系的一个有效的方法,但是较贵的浓度梯度配胶设备限制了该技术的应用,一个简易的装置能够有效地普及这项技术。本文报道了这一简易装置的设计和利用该装置对大豆根化感物质降解细菌的分析效果。 PCR--DGGE is an effective method that molecular biologist use to analyze microorganism diver sity. But expensive equipment limits its application. A simple equipment designed can popularize such technology successfully.
作者 王德国 雷勃钧 张俐俐 刘琦 刘昭军
机构地区 东北农业大学生命科学院 黑龙江省农业科学院生物技术研究中心
出处 《大豆科学》 CAS CSCD 北大核心 2006年第2期195-196,198,共3页 Soybean Science
关键词 浓度梯度凝胶 电泳 装置 大豆根际细菌 Concentration gradient gel Electrophoresis Equipment Soybean rhizosphere bacteria
分类号 Q93-33 [生物学—微生物学]
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  • 1Arsene, F,. Katupitiya, S., Kennedy, I. R. ,and Elmerich, C.Use of lacZ fusions to study the expression of nif genes of Azospirillum brasilense in association with plants[J].Mol.Plant Microbe Interact.1994.7,748 - 757.
  • 2Tenover,F.C. ,and Unger,R.R. Nucleic acid probes for detection and identification of nfectious agents. In “Diagnostic Molecular Microbiology-Principles and Applications” [ M ].(D. H. Persing, T. F. Smith, F. C. Tenover, and T.J.White,eds.) American Society for Mcrobiology, Washington,DC. 1993,223- 228.
  • 3Wilson, K. Preparation of genomic DNA from bacteria. In “Current Protocols in Molecular Biology”[M]. (F. M.Ausubel,ed. ) John Wiley &Sons,New York. 1994 ,Section 2.4.5.
  • 4Threlfall,E.J,Torre.E., Ward,L.R., Davolos - Perez, A.,Rowe, B., and Gibert, I. Insertion sequenoe IS200 finserprinting of Salmonella typhi:An assessment of epidemiological applicability[ J]. Epidem. Infect. 1994,112,253 -261.
  • 5Navarro,. E.,, Simonet, P., Normand, P., and Bardin, R.Characterization of natural populations of Nitrobacter spp. using PCR/RFLP analysis of the ribosomal intergenic spacer[J]. Arch. Microbiol. 1992,157,107-115.
  • 6Hood. D.W. PFGE in the study of a bacterial pathogen(Haemophilus influenzae).In “Pulsed Field Gel Electrophoresis-A Practice Approach”[M]. (A.P.Monaco,ed.)Oxford University Press, Oxford. 1995.
  • 7Muyzer,G. ,de Waal,E.C. ,and Uitterlinden, A. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction- ampli-fied genes coding for 16SrRNA[J]. Appl.Environ.Microbiol.1993,59,695 - 700.
  • 8Sheffield,Y.C. ,Beck,J.S. ,Stone,E.M. ,and Myers, R.M.A simple and efficient method for attaching a 40bp GC -rich sequence to PCR amplified DNA[J]. Biotechniques, 1992,12,386 - 387.
  • 9Loper.J.E,. and Lindow,S.E. Reporter gene systems useful in evaluting in situ gene expression by soil and plant- associated bacteria. In “Manual of Environmental Microbiology ”[M]. (C. J. Hurst, G.R.Knudsen,M.J.Mclnerney,L.D.Stetzenbach, and M. V. Walter, eds. ) American Society for Microbiology Press, Washington,DC.1997, .482-492.
  • 10Buell,C .R, .and Aderson, A.J. Expression of the agg A locus of Pseudomonas putida is in vitro and in plants as detected by the reporter gene,xyIE[J] .Mol.Plant Microbe Interact.1993,6,331 - 340.

共引文献205

同被引文献16

  • 1姚文,朱伟云,韩正康,Antoon D L Akkermans,Barbara Williams,Seerp Tamminga.应用变性梯度凝胶电泳和16SrDNA序列分析对山羊瘤胃细菌多样性的研究[J].中国农业科学,2004,37(9):1374-1378. 被引量：53
  • 2刘新春,吴成强,张昱,杨敏,李红岩.PCR-DGGE法用于活性污泥系统中微生物群落结构变化的解析[J].生态学报,2005,25(4):842-847. 被引量：77
  • 3沈仓良,姚文,陈伟华,朱伟云,邹思湘.伴大豆球蛋白水解肽对灌喂大肠杆菌(E.coli)的小鼠肠道微生物区系和健康的影响[J].中国现代医学杂志,2006,16(3):354-358. 被引量：5
  • 4邢德峰,任南琪.应用DGGE研究微生物群落时的常见问题分析[J].微生物学报,2006,46(2):331-335. 被引量：82
  • 5Zhongtang Yu. Mark Morrison. Comparisons of Different Hypervariable Regions of rrs Genes for Use in Fingerprinting of Microbial Communities by PCR-Denaturing Gradient Gel Electrophoresis[J]. Appl Environ Microbiol, 2004, 70 (8): 4800-4806.
  • 6Reeson AF. Application of 16SrDNA--DGGE to examine the microbial ecology associated with a social wasp vespula gemanica[J]. Inset Mol Biol, 2003,44,231 -- 242.
  • 7Nielsen D S, Moiler P L, Rosenfeldt V, et al. Case study of the distribution of mucosa- associated Bifidobacterium species, Lactobacillus species, and other iacticacid bacteria in the human colon[J]. Appl Environ Microbiol,2003,69(12) :7545- 7548.
  • 8Patrick Butaye, Lue A. Devriese, Freddy Haesebrouck. Antimicrobial Growth Promoters Used in Animal Feed : Effects of Less Well Known Antibiotics on Gram-Positive Bacteria[J]. Clinical Microbiology Reviews, 2003,16(2).
  • 9Johansen C. H. , Bjerrum L,. Finster K, and Pedersen K. Effects of a Campylobaeter jejuni Infection on the Development of the Intestinal Microbora of Broiler Chickens[J]. Poultry Science, 2006,85 : 579-587.
  • 10Pedroso A. A. , Menten J. F. M. , Lambais M. R et al. Intestinal Bacterial Community and Growth Performance of Chickens Fed Diets Containing Antibiotics [J]. Poultry Science, 2006,85 : 747-752.

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大豆科学
2006年 第2期

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