摘要
应用响应面分析法研究温度、乙醇浓度、料液比和时间对柞蚕中β-蜕皮激素提取的影响。以β-蜕皮激素的得率为衡量指标,确定提取的最佳工艺参数为:提取温度80℃、40%乙醇溶液、料液比1∶20、提取时间2.5 h。按此最佳工艺条件提取β-蜕皮激素,用HPD 600型大孔树脂分离、纯化,冷冻干燥得β-蜕皮激素的提取物,经紫外分光光度法测定,β-蜕皮激素的得率为0.0842%。
The effects of temperature, ethanol concentration, the ratio of material to liquid and extraction time on extraction of β-ecdysone from tussore （larva） were studied by response surface methodology. The extraction parameters, according to the yield of β-ecdysone from tussore, were optimized as follows ： the ratio of material to liquid is 1 ： 20, the alcohol concentration is 40%, extracted at 80℃ for 2.5 hours. The extracted β-ecdysone was separated and purified by HPD600 type maeroporous adsorbent resins, desiccated by refrigeration, and mensurated by ultraviolet absorption spectroscopy. As a result, the yield of β-ecdysone was 0. 0842%.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2006年第5期173-175,共3页
Transactions of the Chinese Society of Agricultural Engineering
关键词
柞蚕
β-蜕皮激素
提取
响应面分析
tussore
β-ecdysone
extraction
response surface analysis
