骨髓抑制性贫血小鼠模型的研究

Study on the Model of Medulla-suppressed Anemia in Mice

目的建立适用疗效观察的骨髓抑制性贫血小鼠模型。方法本实验采用BALB小鼠80只,分10组,每组8只,通过正常组、单纯照射组、单纯环磷酰胺注射组、照射加环磷酰胺注射组和照射加环磷酰胺加氯霉素注射组等复合造模比较骨髓造血情况。结果与正常组相比,所有造模组的血像和有核细胞数都显著性降低。根据死亡率和全血细胞数分析,复合造模照射加环磷酰胺组的效果最为突出。结论复合造模照射加环磷酰胺注射组是一种小鼠全血细胞下降骨髓抑制性贫血的理想模型。

Objective To establish an optimal model of medulla-suppressed anemia mice for curative observation. Methods 80 normal BALB/c mice were distributed randomly into 10 groups. The medulla haematogenous effect was compared among the normal group, the irradiation group, the injection cyclophosphamide group, the irradiation and injection cyclophosphamide combation group and the irradiation, cyclophosphamide and chloramphenicol combation group. Results Compared with normal group, the hemogram and bone marrow cell in other groups all decreased singificantly. Through analyzing death rate and amount of whole blood cell, effect of the irradiation and cyclophosphamide combation group was the most remarkable. Conclusion The irradiation and cyclophosphamide combation group is the best model for suppressing hematopoiesis of bone marrow in anemia mice.