实验动物体外寄生虫三种检测方法的比较

Comparison of Three Ectoparasites Inspection Methods on Laboratory Animals

目的对几种实验动物体外寄生虫感染情况进行初步调查,并对三种检测方法进行比较。方法采用拔毛法、刮皮法、处死法三种方法。结果处死法检测四组小鼠样本的体外寄生虫感染率分别是75.5%、90.0%、73.3%、85%,拔毛法和刮皮法分别为19.8%、27.3%、10.0%、75.0%与19.8%、60.6%、40.0%、65.0%。用处死法测得豚鼠体表长虱和新西兰兔体表疥螨的感染率均为100%。结论处死法检测实验动物体表寄生虫效果较好,但处死法与其它两种方法结合,可使检测结果更具科学性。

Objective To compare three methods for inspection of ectoparasites on laboratory animals. Methods Samples were taken by animal sacrifice, pulling up the animal＇ s hair and scraping the animal＇ s skin. Results The infectious rate of mite in mice from four laboratory animal center was 75.5% , 90.0% , 73.3% and 85% , respectively, which was higher than those obtained by the methods of pulling up the animals＇ hair （ 19.8% , 27.3% , 10.0% , 75.0% ） or scraping the animals＇ skin （19.8% , 60.6% , 40.0% , 65.0% ）. The infectious rate of louse in Guinea pigs and mite in New Zealand rabbits was all 100% revealed by sacrifice method. Conclusion Sacrifice method is better than pulling up hair and scraping skin methods, but combination of those two or three methods is the best way to monitor ectoparasites on laboratory animals .